ChloroquineV1, Main, Exploration, bibRecord, 003B46

Ribosomes of the malarial parasite, Plasmodium knowlesi —I. Isolation, activity and sedimentation velocity

Identifieur interne : 003B46 ( Main/Exploration ); précédent : 003B45; suivant : 003B47

Ribosomes of the malarial parasite, Plasmodium knowlesi —I. Isolation, activity and sedimentation velocity

Auteurs : Robert T. Cook [États-Unis] ; Robert C. Rock [États-Unis] ; Masamichi Aikawa [États-Unis] ; Maurille J. Fournier Jr. [États-Unis]

Source :

Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology [ 0305-0491 ] ; 1971.

RBID : ISTEX:6DE04B1D52D7D53EE7165AC0735F09AAF6EC5DD0

English descriptors

Teeft :
- Aliquot, Amino, Amino acid, Amino acid incorporation, Amino acids, Analytical ultracentrifuge, Archs biochem, Bacterial contamination, Bacterial counts, Bacterial ribosomes, Bacterial systems, Biol, Biophys, Buoyancy factors, Chloroquine, Complete system, Electron micrographs, Electron microscopy, Experimental malaria, Experimental pathology, Filter paper blanks, Filtration, Filtration procedure, Final concentration, Glass homogenizer, Graphic representations, Hamilton ruth, Higher concentrations, Higher plants, Incorporation, Incorporation studies, Knowlesi, Large aggregates, Liver ribosomes, Magnesium, Magnesium concentration, Magnesium withdrawal, Malarial, Malarial parasite, Malarial parasites, Malarial pigment, Malarial ribosomes, Many occasions, Molecular weights, National institutes, Other hand, Parasite, Plasmodium, Plasmodium knowlesi, Primary subunits, Protein synthesis, Puromycin, Reaction mixtures, Recent work, Reisner, Reserve university, Ribosomal, Ribosomal fraction, Ribosomal fractions, Ribosomal preparation, Ribosomal preparations, Ribosome, Room temperature, Sedimentation, Sedimentation constants, Sedimentation velocity, Sedimenting material, Several protozoa, Siegel sisler, Small components, Smaller subunits, Special issue, Specific activity, Standard buffer, Subunit, Sucrose gradients, Supernatant, Supernatant factors, Supernatant fractions, Taylor storck, Translation factors, Walter reed army, Walter reed army institute.

Abstract

Abstract: 1.1. An effective procedure for the isolation in good yield of active ribosomes of the malarial parasite Plasmodium knowlesi is described.2.2. The isolated ribosomes have an average monomeric sedimentation velocity of 79 S.3.3. Graded withdrawal of magnesium produces particles of 59 S, 50 S, 44 S, 24 S and 16 S.4.4. From the data obtained and from literature discussed, it is inferred that malarial ribosomes are unlike both bacterial and animal ribosomes. Stringent classification will require extensive study of the ribosomal RNA of the parasites.

Url:

https://api.istex.fr/ark:/67375/6H6-03ZWFJC4-6/fulltext.pdf

DOI: 10.1016/0305-0491(71)90113-1

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 001172
to stream Istex, to step Curation: 001172
to stream Istex, to step Checkpoint: 002886
to stream Main, to step Merge: 003C26
to stream Main, to step Curation: 003B46

Le document en format XML

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<term>Amino acid incorporation</term>
<term>Amino acids</term>
<term>Analytical ultracentrifuge</term>
<term>Archs biochem</term>
<term>Bacterial contamination</term>
<term>Bacterial counts</term>
<term>Bacterial ribosomes</term>
<term>Bacterial systems</term>
<term>Biol</term>
<term>Biophys</term>
<term>Buoyancy factors</term>
<term>Chloroquine</term>
<term>Complete system</term>
<term>Electron micrographs</term>
<term>Electron microscopy</term>
<term>Experimental malaria</term>
<term>Experimental pathology</term>
<term>Filter paper blanks</term>
<term>Filtration</term>
<term>Filtration procedure</term>
<term>Final concentration</term>
<term>Glass homogenizer</term>
<term>Graphic representations</term>
<term>Hamilton ruth</term>
<term>Higher concentrations</term>
<term>Higher plants</term>
<term>Incorporation</term>
<term>Incorporation studies</term>
<term>Knowlesi</term>
<term>Large aggregates</term>
<term>Liver ribosomes</term>
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<term>Magnesium concentration</term>
<term>Magnesium withdrawal</term>
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<term>Malarial parasite</term>
<term>Malarial parasites</term>
<term>Malarial pigment</term>
<term>Malarial ribosomes</term>
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<term>Molecular weights</term>
<term>National institutes</term>
<term>Other hand</term>
<term>Parasite</term>
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<term>Plasmodium knowlesi</term>
<term>Primary subunits</term>
<term>Protein synthesis</term>
<term>Puromycin</term>
<term>Reaction mixtures</term>
<term>Recent work</term>
<term>Reisner</term>
<term>Reserve university</term>
<term>Ribosomal</term>
<term>Ribosomal fraction</term>
<term>Ribosomal fractions</term>
<term>Ribosomal preparation</term>
<term>Ribosomal preparations</term>
<term>Ribosome</term>
<term>Room temperature</term>
<term>Sedimentation</term>
<term>Sedimentation constants</term>
<term>Sedimentation velocity</term>
<term>Sedimenting material</term>
<term>Several protozoa</term>
<term>Siegel sisler</term>
<term>Small components</term>
<term>Smaller subunits</term>
<term>Special issue</term>
<term>Specific activity</term>
<term>Standard buffer</term>
<term>Subunit</term>
<term>Sucrose gradients</term>
<term>Supernatant</term>
<term>Supernatant factors</term>
<term>Supernatant fractions</term>
<term>Taylor storck</term>
<term>Translation factors</term>
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<front><div type="abstract" xml:lang="en">Abstract: 1.1. An effective procedure for the isolation in good yield of active ribosomes of the malarial parasite Plasmodium knowlesi is described.2.2. The isolated ribosomes have an average monomeric sedimentation velocity of 79 S.3.3. Graded withdrawal of magnesium produces particles of 59 S, 50 S, 44 S, 24 S and 16 S.4.4. From the data obtained and from literature discussed, it is inferred that malarial ribosomes are unlike both bacterial and animal ribosomes. Stringent classification will require extensive study of the ribosomal RNA of the parasites.</div>
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Ribosomes of the malarial parasite, Plasmodium knowlesi —I. Isolation, activity and sedimentation velocity

Ribosomes of the malarial parasite, Plasmodium knowlesi —I. Isolation, activity and sedimentation velocity

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri